Boost Your Probiotic Product Development

Offering

Impact

Cooperation

Platform

Metrics

Applications

Partner with us and leverage Differential Bio’s Virtual Scale-up Platform to boost yields, cut costs, and confidently scale probiotic production with the power of robotics and AI.

Start Your Optimization

•Offering

Accelerating Probiotic Strain Development

We combine robotic data generation with AI to identify high-potential microbes and accelerate their path to market. Our Probiotic Optimization Pipeline screens thousands of media–strain combinations to find top performers, enabling growth, manufacturability, and regulatory compliance, from lactic acid bacteria to strict anaerobic gut microbes, while maximizing stability and functional activity.

Screening &
Characterization

Select probiotic strains with desired functions for anaerobic and aerobic fermentation using automated HTS data generation.

Process Development

Adjust pH, temperature, and aeration to maximize yield, temperature- and process stability (for instance, lyo-/cryoprotection).

Formulation

Tailor formulations for cost efficiency and functional performance for e.g., animal-free, allergen-free manufacturing, while preserving viability.

Scale Up

With the identified strain and optimized parameters, the bioprocess is ready for efficient scale-up to industrial production.

•Optimisation

Turning Probiotic Complexity into Scalable Success

Maximize probiotic process productivity without compromising strain functionality, all through medium optimisation. Our platform screens thousands of media permutations to balance complex objectives, enabling both product optimisation and commercial success far beyond what manual methods allow.

•Boost

Enhance probiotic productivity by pinpointing optimal media compositions, driving higher yields and viable cell counts for large-scale manufacturing.

•Protect

Ensure cell viability and stability across USP, DSP, and formulation by fine-tuning nutrients, conditions, and protective blends, safeguarding potency, freeze-dry survival, and shelf-life.

•Comply

Design compliant, consumer-ready formulations, allergen-free, animal-free, or plant-based, ensuring alignment with standards, reduced regulatory risk, and smoother market entry.

CFU Yield

Maximize viable cell output with higher CFU counts per batch.

Revenue

Enhance product titer and yield, enabling higher-value outputs and scalable production.

Cost

Increase economic viability, enabling fewer runs, with less ingredients and reduced COGS.

Other

Tailored optimisation for product purity, stability, and other process-specific needs

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•Optimisation

Turning Probiotic Complexity into Scalable Success

•Boost

Enhance probiotic productivity by pinpointing optimal media compositions, driving higher yields and viable cell counts for large-scale manufacturing.

•Protect

Ensure cell viability and stability across USP, DSP, and formulation by fine-tuning nutrients, conditions, and protective blends, safeguarding potency, freeze-dry survival, and shelf-life.

•Comply

Design compliant, consumer-ready formulations, allergen-free, animal-free, or plant-based, ensuring alignment with standards, reduced regulatory risk, and smoother market entry.

CFU Yield

Maximize viable cell output with higher CFU counts per batch.

Revenue

Enhance product titer and yield, enabling higher-value outputs and scalable production.

Cost

Increase economic viability, enabling fewer runs, with less ingredients and reduced COGS.

Other

Tailored optimisation for product purity, stability, and other process-specific needs

LEarn more

•Why Differential Bio

Unlock the Power of Your Probiotic

Supercharged screening, in-silico first: Together with you, we combine robotic data generation, ML-based fermentation prediction, and 10× faster hypothesis testing to minimize costly large-scale trial runs and achieve better fermentation outcomes compared to manual workflows.

Better Outcomes

Efficient, sustainable process conditions that cut operating costs, boost CFU counts, and reduce production losses from seed to shelf.

Quick R&D ROI

Projects pay for themselves within the first three batches, unlocking ROI through higher yields and lower costs.

Faster Time to Market

The Probiotic Optimization Pipeline shorten development cycles, testing thousands of media compositions in weeks for faster scale-up with lower risk.

•Case Study

Case Study: Boosting Yield and Efficiency

We partnered with CanBiocin, a leader in probiotics for premium pet foods and feed that support digestive and immune health. In just four weeks, the AI- and robotic-guided optimization cycle uncovered several high-potential reformulation strategies for CanBiocin’s scaled-up Limosilactobacillus reuteri strain. The selected “value-for-money” formulation improved unit economics and validated AI-guided media design as a strategic lever in their biomanufacturing roadmap.

READ FULL CASE STUDY

Run Scale

Let's collaborate

Partner with Differential Bio to define the key medium components and process parameters that drive your optimisation. Leverage our secure in-house HTS platform, always with full IP protection.

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•Bottlenecks

Our Solution for Next-Generation Probiotics

Next-generation probiotics offer enormous potential, but each strain brings hurdles, from high cell density to unique nutrient needs and stability. Traditional methods struggle under anaerobic conditions or with hard-to-culture species. Our Virtual Scale-up Platform unlocks this potential by combining automated data generation and AI modeling to systematically develop, optimize, and commercialize even the most challenging NGP strains.

More Data

We capture reliable growth data even in opaque or turbid media using robotic HTS and tailored assays, generating high-quality datasets where conventional methods fail.

Modeled Organisms

Our AI-driven microbial models transform complex experimental data into predictive insights , eliminating years of trial-and-error and accelerating the path to scalable conditions.

Beyond LAB

We empower your probiotic development beyond “easy” lactic acid bacteria, enabling robust growth strategies for demanding gut species and novel strains with unique health benefits.

• How it Works

We Scale Down to Scale Up

Step One

HTS Data Acquisition

By mimicking large-scale stress factors in a microliter-scale environment, we gather industrially relevant data as affordably as possible.

Step Two

AI-Based Modeling

Our specialized models deploy AI to make accurate predictions from wet-lab data such as biomass growth or protein expression.

Step Three

Domain-Specific Application

We drive fermentation outcomes using verticalized AI models, seamlessly integrating these tools into bioprocess R&D workflows.

•Technology

BioProcess Optimization Hub

Collected data, the in-silico model is then developed to simulate the full spectrum of conditions. You can access this model through our web-based platform to run unlimited virtual experiments, enabling efficient optimization.

•Metrics

Aligning Biology with Economics through Measurable Impact

Define your optimization objective—such as growth, yield, or cost—and we design targeted experiments and build predictive models to simulate and optimize the corresponding bioprocess parameters.

Increase revenue

Maximize revenue by optimizing bioprocess parameters that enhance product titer and yield, enabling higher-value outputs and efficient scalability.

Reduce cost

Minimize production costs by optimizing bioprocess configurations that reduce resource consumption while sustaining high yield and performance.

Product quality

Optimize nutrients, stress factors, and cultivation parameters to maintain high cell viability and improve product quality from a healthy, productive culture.

•Applications